MICROBIOLOGY: PRACTICE 2

SINGLE BEST OPTION SERIES

         INSTRUCTION: SELECT THE SINGLE BEST OPTION

1.            During sterilization in microbiology laboratory, since heat is less effective on dry material, it is customary to apply a temperature of 160 to 170⁰C for at least...................
   a.       60 minutes
   b.      5  minutes
   c.       30 minutes
   d.      40 minutes
   e.       35 minutes
   2.       Heat sensitive medical devices can be effectively sterilized by vapour-phase systems employing the following except:
   a.       Ethylene oxide
   b.      Chlorhexidine
   c.       Hydrogen peroxide
   d.      Peracetic acid
   e.      Formaldehyde
   3.       Which of the following is incorrect about the activity of povidone iodine:
   a.       Virucidal
   b.      Fungicidal
   c.       Bacteriostatic
   d.      Tuberculocidal
   e.      Sporicidal
   4.       Which of the following is incorrect about hydrogen peroxide in health industry:
   a.       Has a broad spectrum activity against viruses, bacteria and bacterial spores
   b.      Sporicidal activity requires concentration of 10% or more.
   c.       Sporicidal activity requires longer contact time
   d.      It has no activity against yeasts 
   e.      None of the above
   5.       Which of the following is incorrect about phases of the bacterial growth curve:
   a.       Lag phase growth rate is zero
   b.      Growth rate at maximum stationary phase is constant
   c.       Growth rate at the exponential phase is constant
   d.      Growth rate at decline phase is negative
   e.      None of the above
   6.       The average time required for bacteria population to double in a culture, is referred to as:
   a.       Generation time
   b.      Culture time
   c.       Log time
   d.      Decline time
   e.      Maximum stationary phase
   7.       Which of the following is incorrect about disinfectants?
   a.       They render a surface free of viable micro-organisms
   b.      They reduce the number of viable micro-organisms
   c.       They are not necessarily sporicidal
   d.      Povidone iodine is a disinfectant
   e.      None of the above
   8.       Penicillin generally act by:
   a.       Destroying the cell wall
   b.      Preventing the synthesis of cell wall
   c.       By removal of sulfhydryl group
   d.      Protein denaturation
   e.      Antagonism of energy yielding processes.
   9.        A 36 year old patient has an abscess with a strain of Staphylococcus aureus that is beta-lactamase positive. This factor indicates that the organism is resistant to which of the following antibiotics?
   a.       Penicillin G, ampicillin and piperacillin
   b.      Trimethoprim-sulphamethoxazole
   c.       Erythromycin, clarithromycin and azithromycin
   d.      Vancomycin
   e.      None of the above
   10.   Which of the following regarding fungi is correct?
   a.       All fungi are able to grow as yeast and molds
   b.      Although fungi are eukaryotes, they lack mitochondria
   c.       Fungi are photosynthetic
   d.      Fungi have one or more nucleic acid chromosome
   e.      Few fungi posses cell membrane 
   
   
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